Applications
Interaction proteomics
Map protein–protein interactions and complexes.
A protein's function is written in the company it keeps. Knowing a gene is expressed tells you little about the complex it assembles into or the partners it recruits under a given condition. We resolve those interactions with affinity- and proximity-based workflows read out by high-resolution MS, and use proper controls and statistics to tell specific interactors from the sticky background that plagues pulldowns.
Example projects
Typical study designs
- AP-MS / co-IP interactome mapping
- Proximity-labeling interactomes (BioID, APEX)
- Complex composition and stoichiometry
- Condition-dependent interaction changes
- Bait-based interactor discovery with controls
Let's plan your study
Every study that is executed incorrectly consumes valuable sample material, and, more importantly, wastes time and manpower. In our fast-paced scientific environment, these resources cannot be recovered.